Figure 3
Validation of G6PD as a novel miR-122 target. (a) Luciferase reporter assay of G6PD 3′-UTR (wild type or mutant) driven Renilla luciferase activity normalized to firefly luciferase after transfection H293T cells with scrambled (NC) or miR-122 mimic RNA (miR-122). (b,c) G6PD mRNA levels in HCC cells transfected with in miR-122 mimic or scrambled (NC) RNA (b), and in Huh-7 cells transfected with miR-122 anti-sense oligo (miR-122 KD) or negative control oligo (NC) (c). mRNA levels were measured by RT-qPCR.
