Figure 4
Cells expressing KRASG12V were highly glycolytic and accumulated lactic acid. (A) CaCO2-BRAFV600E and CaCO2-KRASG12V cells were exposed to 1.0 g/L of 13C-glucose for 5 min, harvested and measured with GC-MS. For protein abundance, cells were measured with LC-MS. The mean ratio (log 2 fold changes, fc) of CaCO2-KRASG12V to CaCO2-BRAFV600E for labeled metabolites and z-score of relative protein quantities are shown. (B) Mathematical model with the fluxes for each reaction. Species with rounded corners are species where concentration data was available. On the right side (or above) of edges are the reaction names, on the left side (or below) of the edges are the flux values of the reaction. The gray shaded node is an external species. Flux values are in µmol/s. 3PG: 3-phosphoglycerate, Glc6P: glucose-6-phosphate, HK: hexokinase, Lac: lactic acid, PGK: phosphoglyceratekinase, PSP: phosphoserine phosphatase, Pyr: pyruvic acid, Ser: serine. glc: glucose.
