Figure 1

mRNA and protein expression levels of WNK1 in mouse skeletal muscle and differentiating C2C12 mouse skeletal muscle cells. (A) Reverse transcription polymerase chain reaction for four mammalian WNK isoforms WNK1, WNK2, WNK3, and WNK4 in C2C12 pre-differentiation myoblasts, differentiated myotube, mouse quadriceps, heart, and kidney. Only WNK1 was abundantly expressed in both C2C12 cells and skeletal muscle tissue. (B) Immunoblots for WNK1 and myogenic markers in differentiating C2C12 cells. WNK1 expression was increasingly upregulated during differentiation in parallel with the increasing expression of the myogenic markers myogenin and myosin heavy chain. MHC, myosin heavy chain; WNK, with-no-lysine (K). Full-length blots/gels are presented in Supplementary Fig. 7.