Figure 3
WNK1 silencing increased FOXO4 nuclear localization in C2C12 cells. Immunoblots (left) and densitometric analyses (right) of three FOXO isoforms, namely FOXO1, FOXO3, and FOXO4, as well as Smad2/3 after separating nuclear (upper)/cytoplasmic (lower) extracts in WNK1-silenced C2C12 cells. FOXO4 levels were selectively increased in the nuclei of C2C12 cells treated with WNK1-targeted siRNA. The hash (#) shows FOXO4. Values are presented as the mean ± standard error of the mean (n = 4 or 7 per experimental group). *P < 0.05 versus the control cells. Full-length blots are presented in Supplementary Fig. 9.
