Figure 6

WNK1 was not co-immunoprecipitated with FOXO4. Halo-tagged human WNK1 was co-immunoprecipitated with FLAG-tagged human KLHL3, but not with human FOXO4, in HEK293T (left) and C2C12 cells (right). WNK1, with-no-lysine (K); FOXO4, Forkhead box protein O4; kelch like family member 3, KLHL3. Full-length blots are presented in Supplementary Fig. 12.