Table 2 Expression stability ranking of the candidate reference genes according to BestKeeper, NormFinder and geNorm original softwares and the Delta CT and RefFinder analysis.
Ranking | BestKeeper | NormFinder | GeNorm | Delta CT analysis | RefFinder analysis | |||||
|---|---|---|---|---|---|---|---|---|---|---|
Gene | r (p-value) | Gene | Stability Value (standard error) | Gene | M value | Gene | Average of STDEV | Gene | Geometric mean of ranking values | |
1 | recA | 0,849 (0.001) | recA | 0,296 (0.082) | rpoC | 0,44 | recA | 0,92 | recA | 1.78 |
2 | rho | 0,816 (0.001) | rho | 0,328 (0.085) | proC | 0,44 | rho | 0,92 | rpoD | 2.99 |
3 | glnA | 0,814 (0.001) | proC | 0,423 (0.096) | rpoD | 0,49 | rpoD | 1 | rho | 3 |
4 | gyrA | 0,739 (0.001) | rrsH | 0,515 (0.109) | rrsH | 0,6 | gyrA | 1,01 | rpoC | 3.13 |
5 | ffh | 0,712 (0.002) | gyrA | 0,535 (0.112) | recA | 0,67 | proC | 1,02 | proC | 3.31 |
6 | rpoD | 0,687 (0.003) | rpoC | 0,614 (0.124) | rho | 0,78 | rpoC | 1,06 | rrsH | 4.28 |
7 | proC | 0,66 (0.005) | rpoD | 0,674 (0.134) | gyrA | 0,86 | rrsH | 1,08 | gyrA | 6.19 |
8 | rpoC | 0,541 (0.03) | ffh | 0,76 (0.148) | glnA | 0,92 | glnA | 1,12 | glnA | 7.74 |
9 | rrsH | 0,42 (0.106) | glnA | 0,776 (0.15) | ffh | 0,99 | ffh | 1,31 | ffh | 9 |
