Table 2 Results of the primer-probe specificity test with genomic DNA using real-time multiplex polymerase chain reaction.

From: Real-time multiplex PCR for simultaneous detection of multiple species from environmental DNA: an application on two Japanese medaka species

Primer probe set

genomic DNA template (0.1 ng)

PCR positive (repeatability)

latipes

sakaizumii

Both (real-time multiplex PCR)

latipes

1

3/3 (0.11)

0/3

2

3/3 (0.11)

0/3

3

3/3 (0.17)

0/3

sakaizumi

1

0/3

3/3 (0.10)

2

0/3

3/3 (0.07)

3

0/3

3/3 (0.44)

latipes 1 and sakaizumii 1

3/3 (0.19)

3/3 (0.14)

latipes 2 and sakaizumii 2

3/3 (0.09)

3/3 (0.01)

latipes 3 and sakaizumii 3

3/3 (0.22)

3/3 (0.12)

mosquitofish

0/3

0/3

guppy

0/3

0/3

  1. The identification numbers of the genomic DNA template indicate different individuals. The repeatability of three PCR replications are shown as SD of Ct values.
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