Figure 4
From: β-Oxidation in ghrelin-producing cells is important for ghrelin acyl-modification
Effect of inhibiting β-oxidation on ghrelin production in PG-1 cells. PG-1 cells were plated in 24-well plates and etomoxir (ET, 5 µmol/L), octanoate (OA, 50 µmol/L), or a mixture of stearate and palmitate (LA, 50 µmol/L) was added. After incubation, acyl-ghrelin levels (a), des-acyl ghrelin levels (b), and acyl/total ghrelin ratio (c) in the medium were measured. Des-acyl ghrelin levels were not different between groups. ET treatment decreased acyl-ghrelin levels and the acyl/total ghrelin ratio, and ET + OA rescued acyl-ghrelin and acyl/total ghrelin ratio (a–c). On the other hand, Ghrl and Mboat4 mRNA expression was not changed between all experimental groups (d,e). PG-1 cell morphology did not change after the etomoxir treatment (f). This experiment was replicated twice and the results of 2nd data set were used in this figure. Scale bars=500 μm Each value represents the mean ± SEM. n = 3.
