Figure 5
Comparison of OsAPX2 fractions and molecular switching of OsAPX2 in response to salt treatment and recovery. Each fraction was analysed by western blotting with polyclonal anti-OsAPX2 antibodies after separation using 10% native-PAGE (upper panel) or 12% SDS-PAGE under reducing conditions (lower panel). (a) Structural analysis of fractionated OsAPX2 proteins by western blotting. The OsAPX2 proteins were separated into four fractions corresponding to the major dimeric form (F4), the LMW forms (F2 and F3), and the HMW complexes (F1). (b) Molecular switching of OsAPX2 in response to salt-stress treatment and recovery in IR29. Rice seedlings were cultivated in 0.5× Murashige and Skoog (MS) liquid medium for 3 weeks (Lane 1). For salt stress, 3-week-old cultivated rice plants were transferred to nutrient solution (0.5× MS medium) containing 100 mM NaCl for 3 d (Lane 2). For recovery, rice plants treated with salt stress were transferred to a new nutrient solution containing no salt and grown for 3 d (Lane 3).
