Figure 6 | Scientific Reports

Figure 6

From: ADAM-like Decysin-1 (ADAMDEC1) is a positive regulator of Epithelial Defense Against Cancer (EDAC) that promotes apical extrusion of RasV12-transformed cells

Figure 6

Knockdown of ADAMDEC1 suppresses filamin accumulation in normal cells and EPLIN accumulation in RasV12-transformed cells at their interface. (a,b) MDCK-pTR GFP-RasV12 cells were co-cultured with MDCK or MDCK ADAMDEC1-shRNA1 cells. Cells were fixed at 16 h after tetracycline addition and stained with anti-filamin antibody (red). (a) Confocal microscopic immunofluorescence images of filamin. (b) Quantification of filamin accumulation. Data are mean ± SD from five independent experiments. (c,d) MDCK-pTR GFP-RasV12 cells were co-cultured with MDCK or MDCK ADAMDEC1-shRNA1 cells. Cells were fixed at 16 h after tetracycline addition and stained with anti-EPLIN antibody (red) and Hoechst (blue). (c) Confocal microscopic immunofluorescence images of EPLIN. (a,c) Scale bars, 10 μm. (d) Quantification of EPLIN accumulation. Data are mean ± SD from three independent experiments. Values are expressed as ratios relative to MDCK: RasV12 = 50:1. *P < 0.05, two-tailed Student’s t-test.

Back to article page