Figure 2 | Scientific Reports

Figure 2

From: LncRNA expression profiling of BMSCs in osteonecrosis of the femoral head associated with increased adipogenic and decreased osteogenic differentiation

Figure 2

The adipogenic differentiation ability of BMSCs between the SONFH (n = 16) and control groups (n = 16). (A) The SONFH group had stronger Oil Red O staining (40× and 200×) than the control group. (B) For immunohistochemical staining of adipogenic markers (PPARγ, C/EBPα), the SONFH group had stronger staining than the control group. (C) The Oil Red O quantification of SONFH group was significantly higher than that of the control group at day 10 and 14. (D) The number of cells with Oil Red O staining (100×) in SONFH group was significantly higher than control group at day10 and day 14. (E) The expression levels of PPARγ, C/EBPα and Adipsin during adipogenic differentiation of BMSCs in the SONFH group were significantly increased comparing to the control group. All samples were normalized to the expression of GAPDH, and the relative expression levels of each gene were analyzed using the 2−△△Ct method. *P < 0.05, **P < 0.01.

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