Figure 2 | Scientific Reports

Figure 2

From: Direct current stimulation of endothelial monolayers induces a transient and reversible increase in transport due to the electroosmotic effect

Figure 2

Effect of reversing DCS polarity on water flux. (A) Bubble displacement data from single experiment. bEnd.3 monolayers were first exposed to a 10 cm H2O hydrostatic pressure gradient to establish the baseline Jv. DCS (1 mA) was then applied at t = 3600 sec with the cathode on top inducing an immediate decrease in flux that persisted for the 10 min duration of DCS. After reestablishing the baseline for 3600 sec, DCS was applied with the anode on top (t = 7200 sec) inducing an immediate increase in flux that persisted for the duration of DCS. (B) Average Jv as a function of time calculated from the raw data at intervals of 300 sec for n = 5 experiments performed as in (A). Jv significantly decreased or increased with DCS application in the B-A or A-B mode, respectively. *p = 0.007 for B-A mode and p = 0.0002 for A-B mode vs baseline 5 minutes before DCS application by paired t-test.

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