Figure 3
Effect of DCS current magnitude on water flux. (A) Average Jv as a function of time for n = 6 experiments performed as follows: bEnd.3 monolayers were first exposed to a 10 cm H2O hydrostatic pressure gradient to establish the baseline Jv. DCS was then applied at t = 3600 sec at a current of 0.5 mA for 10 min. After reestablishing the baseline for 30 min, DCS was applied at a current of 1 mA for 10 min. Finally, the baseline was reestablished for 30 min and DCS was applied at a current of 1.5 mA for 10 min. (B) DCS-induced flow (JvDCS) was found to be linearly correlated with the magnitude of the applied current density (Ji). Dotted lines show 95% confidence interval; R2 = 0.98. *p = 1.8 × 10−5 for 0.5 mA; p = 1.9 × 10−7 for 1 mA; p = 1.4 × 10−6 for 1.5 mA vs baseline 5 minutes before DCS application by paired t-test.
