Figure 1
An illustration depicting the initial screening criteria. A benchmark line was set at two fold the initial optical density at an absorbance of 595 nm (OD595nm). Our operational definitions of inhibition by the antimicrobial compounds were as follows. ‘Mild or no inhibition’ was defined when the culture reached the benchmark line within 2 to 3 hrs. ‘Inhibitory’ was defined when the benchmark line was reached within 3 to 6 hrs and ‘highly inhibitory’ within 6 to 8 hrs. ‘No outgrowth’ was defined when no increase in OD595nm was reached within 8 hrs. ‘No germination’ was demarcated when the OD595nm did not lower the initial OD595nm in time. Samples that did not show outgrowth after 8 hours were followed for maximally 48 hours.
