Table 3 Live imaging results after treatment of heat activated dormant spores with the synthetic compounds.

From: Evaluating novel synthetic compounds active against Bacillus subtilis and Bacillus cereus spores using Live imaging with SporeTrackerX

Treatment

Conc. (µg/ml)

Start of germination (min)

Germination duration (min)

Burst (min)

Generation time (min)

Bacillus subtilis

Median

p-value

IQR

Counts

Median

p-value

IQR

Counts

Median

p-value

IQR

Counts

Median

p-value

IQR

Counts

Untreated

—

14

—

10

155

4

—

2

155

85

—

17

147

36

—

7

135

C1

21

17

≤0.01

19

188

3

≤0.01

1

188

        

5

14

0.65

10

112

2

0.28

2

112

131

≤0.01

38

102

55

≤0.01

12

90

C2

20

14

0.95

7

213

3

0.87

1

213

        

5

14

0.46

7

137

3

≤0.01

1

137

109

≤0.01

36

68

49

≤0.01

22

59

C3

54

15

0.09

13

223

3

≤0.01

1

223

        

3

14

0.86

9

210

3

≤0.01

2

210

104

≤0.01

26

189

49

≤0.01

17

177

C4

75

15

0.07

10

250

4

≤0.01

2

250

        
 

5

14

0.34

10

213

3

≤0.01

1

213

119

≤0.01

31

148

43

≤0.01

9

175

C5

6.4

14

0.18

12

250

3

≤0.01

2

250

        

1.6

14

0.71

7

212

3

≤0.01

1

212

99

≤0.01

24

199

38

≤0.01

3

187

Bacillus cereus

                

Untreated

—

4

—

1

118

1

—

1

118

75

—

14

111

37

—

3

101

C1

15

4

≤0.01

3

166

2

≤0.01

1

166

        

4

3

0.44

1

164

2

0.03

1

164

73

0.67

29

160

35

≤0.01

8

156

C2

10

3

≤0.01

1

265

2

≤0.01

1

265

        

1

3

≤0.01

2

130

2

≤0.01

2

130

83

≤0.01

25

103

38

≤0.01

9

87

C3

100

9

≤0.01

80

91

2

≤0.01

1

91

        

6

3

0.29

1

155

2

≤0.01

1

155

70

≤0.01

23

109

36

≤0.01

3

99

C4

30

3

≤0.01

1

224

3

≤0.01

2

224

        

4

3

≤0.01

1

107

2

≤0.01

1

107

116

≤0.01

36

93

43

≤0.01

7

84

C5

1.8

4

≤0.01

1

192

2

≤0.01

1

192

        

0.9

4

0.19

4

192

2

≤0.01

1

192

77

0.23

35

98

43

≤0.01

9

77

  1. IQR refers to inter-quartile range.