Figure 3

Nesfatin-1 and TPH immunoreactivity in the DRN of MS and NH rats. (A) Photomicrographs (100 × magnification) of nesfatin-1 and TPH-immunopositive cells in the DRN of MS and NH rats; A1: MS–nesfatin-1; A2: MS–TPH; A3: NH–nesfatin-1; and A4: NH–TPH. (B) Number of nesfatin-1-immunopositive cells in the DRN of MS and NH rats. (C) Number of TPH-immunopositive cells in the DRN of MS and NH rats. MS rats showed significantly elevated numbers of nesfatin-1- and TPH-immunopositive cells in the DRN [*p < 0.05] (n = 6 for each group).