Figure 7
5-HT and TPH immunoreactivity in the DRN of NH rats treated with nesfatin-1 or vehicle. (A) Representative examples of sections processed for 5-HT or TPH immunostaining from NH rats microinjected with nesfatin-1 or vehicle into the DRN; A1: NH + nesfatin-1–5-HT; A2: NH + nesfatin-1–TPH; A3: NH + vehicle–5-HT; and A4: NH + vehicle–TPH. (B) Number of 5-HT-immuno-positive cells in the DRN after nesfatin-1 or vehicle microinjection. (C) Number of TPH-immunoreactive cells in the DRN after nesfatin-1 or vehicle microinjection. Nesfatin-1 microinjection caused a significant elevation in the number of 5-HT and TPH-immunoreactive cells in the DRN of NH rats [*p < 0.05] (n = 6 for each group).
