Table 2 Species associated with beehives and nitidulid beetles for DNA barcoding and tested in the real-time Aethina tumida PCR assay.
Species ID* (Family) | Voucher ID** | n | Origin | Year | Life stage | Origin | Match to SHB, %*** | PCR results | BOLD/GenBank Accession # |
|---|---|---|---|---|---|---|---|---|---|
Aethina tumida Murray (Nitidulidae) | VAITC5359-5366 | 9 | Australia | 2015 | adult | Beehive | 99–100 | + | ANZN012-17 to ANZN018-17 |
AQ3 | 2 | Australia | 2014 | adult | Intercepted | + | ANZN002-17 | ||
AQ4 | 1 | USA | 2015 | larva | Beehive | + | ANZN001-17 | ||
T17_01583A | 5 | Italy | 2015–2016 | adult | Beehive | 94–96 | + | ANZN026-17 to ANZN029-17 | |
T17_01583B | 5 | Italy | 2016–2017 | larva | Beehive | 94–96 | + | ANZN030-17 to ANZN033-17 | |
Aethina concolor (Macleay) (Nitidulidae) | T16_01444 | 1 | New Zealand | 2016 | adult | Beehive | 81–82 | − | ANZN006-17 |
T17_00549 | 1 | New Zealand | 2017 | adult | Collection | − | ANZN008-17 | ||
AQ5 | 1 | New Zealand | 2017 | adult | Collection | − | ANZN007-17 | ||
Brachypeplus sp. (Nitidulidae) | VAITC5397–5399 | 3 | Australia | 1999/2003 | adult | Beehive | − | ANZN021-17 to ANZN023-17 | |
Brachypeplus sp. (Nitidulidae) | 20/09/1992 | 1 | Hawaii | 1992 | adult | Intercepted | n/a | − | ns |
Brachypeplus sp. (Nitidulidae) | Australia 18401 | 1 | Australia | — | larva | Intercepted | n/a | − | ns |
T16_02506 | 1 | Australia | 2016 | adult | Intercepted | 85 | − | ANZN003-17 | |
Carpophilus dimidiatus (Fabricius) (Nitidulidae) | T11_3082 | 1 | China | 2011 | adult | Intercepted | n/a | − | ns |
Carpophilus gaveni Dobson (Nitidulidae) | T11_02455 | 1 | New Zealand | 2011 | adult | Pyrus sp. | n/a | − | ns |
Carpophilus hemipterus (Linnaeus) (Nitidulidae) | VAITC5395 | 1 | Australia | 2003 | Beehive | nt | |||
Carpophilus mutilatus Erichson (Nitidulidae) | 09/2007/455 | 1 | Fiji | 2007 | adult | Intercepted | n/a | − | ns |
Carpophilus oculatus Murray (Nitidulidae) | 09/2009/3046 | 1 | Tonga | 2009 | adult | Intercepted | n/a | − | ns |
Carpophilus sp. (Nitidulidae) | T16_204A | 1 | New Zealand | 2016 | adult | Citrus sp. | n/a | − | ns |
Carpophilus sp. (Nitidulidae) | T16_207A | 1 | New Zealand | 2016 | adult | Citrus sp. | n/a | − | ns |
Dactylosternum abdominale (Fabricius) (Hydrophilidae) | VAITC5375 | 1 | Australia | 2015 | adult | Beehive | n/a | − | ns |
Dermestes ater De Geer (Dermestidae) | VAITC5376 | 1 | Australia | 2015 | adult | Beehive | n/a | − | ns |
Epuraea signata Broun (Nitidulidae) | T16_00204B/207B | 2 | New Zealand | 2016 | adult | Citrus sp. | 82–83 | − | ANZN009-17 to ANZN010-17 |
VAITC5428 | 1 | Australia | 2015 | adult | Beehive | 82–83 | − | ANZN024-17 | |
Epuraea sp. (Nitidulidae) | VAITC5429 | 1 | Australia | adult | Beehive | 82–83 | − | ANZN025-17 | |
Epuraea sp. (Nitidulidae) | T14_03072 | 2 | 2014 | adult | Beehive | 83–84 | − | ANZN004-17 to ANZN005-17 | |
Urophorus humeralis (Fabricius) (Nitidulidae) | 09/2009/27032 | 1 | Tonga | 2009 | adult | Intercepted | n/a | − | ns |
T16_207C | 1 | New Zealand | 2016 | adult | Citrus sp. | 84 | − | ANZN011-17 | |
VAITC5373–5374 | 2 | Australia | 2015 | adult | Beehive | 84 | − | ANZN019-17 to ANZN020-17 | |
Apis mellifera ligustica Spinola (Apidae) | T15_06598 | 2 | New Zealand | 2015 | adult | Beehive | n/a | − | ns |
Varroa destructor Anderson & Trueman (Varroidae) | AV1/AV2 | 2 | New Zealand | 2016 | adult | Beehive | 67–68 | − | MG793455 |
Acarapis woodi (Rennie) (Tarsonemidae) | AQ1 | 1 | Canada | 2015 | adult | Beehive | 67–68 | − | MG793456 |
Acarapis externus Morgenthaler (Tarsonemidae) | Ae52/Ae126 | 2 | New Zealand | 2016 | adult | Beehive | 67–68 | − | MG793457 |
Acarapis dorsalis Morgenthaler (Tarsonemidae) | AQ7 | 1 | New Zealand | 2015 | adult | Beehive | 68–69 | − | MG793458 |
