Table 3 Blind panel tests for the real-time PCR assay against Aethina tumida.

From: Molecular detection of small hive beetle Aethina tumida Murray (Coleoptera: Nitidulidae): DNA barcoding and development of a real-time PCR assay

Sample#

Species

Family

Year

Origins

PCR results (Cq)

BA1

Carpophilus sp.

Nitidulidae

2016

Auckland, NZ

0

BA2

Aethina tumida

Nitidulidae

2015

VIC, Australia

21.2

BA3

Epuraea signata

Nitidulidae

2016

Auckland, NZ

0

BA4

Urophorus humeralis

Nitidulidae

2015

VIC, Australia

0

BA5

Aethina tumida

Nitidulidae

2015

VIC, Australia

23.6

BA6

Carpophilus sp.

Nitidulidae

2016

Auckland, NZ

0

BA7

Acarapis externus

Tarsonemidae

2014

NZ

0

BA8

Epuraea signata

Nitidulidae

2016

Auckland, NZ

0

BA9

Dactylosternum abdominale

Hydrophilidae

2015

VIC, Australia

0

BA10

Brachypeplus sp.

Nitidulidae

2015

VIC, Australia

0

BA11

Carpophilus sp.

Nitidulidae

2016

Auckland, NZ

0

BA12

Apis cerana

Apidae

2015

NZ

0

BA13

Aethina tumida

Nitidulidae

2014

USA

18.3

BA14

Apis mellifera mellifera/ligustica

Apidae

2015

NZ

0

BA15

Apis cerana cerana

Apidae

2013

China

0

BA16

Aethina tumida

Nitidulidae

2014

Australia

25.4

  1. Note: Cq: The cycle at which the fluorescence of a sample first cross the threshold line (ie exceeds background level). It is a measure for a positive reaction in real-time PCR reaction.