Figure 3
From: Chemotherapy-Induced Tunneling Nanotubes Mediate Intercellular Drug Efflux in Pancreatic Cancer
TNT formation amongst pancreatic cancer cells. (A) Top row: Representative images of TNTs connecting pancreatic adenocarcinoma S2013, CAPAN-1, CAPAN-2, MIA PaCa-2, and human pancreatic ductal epithelial (HPDE) cells. Images were taken using an Olympus IX70 inverted microscope. Scale bar = 200 μm. Bottom row: The median number of TNTs/cell (TNT index) over time for the S2013, CAPAN-1, CAPAN-2, MIA PaCa-2, and HPDE cell lines. Individual data points are depicted as shown, with solid lines representing median values. (B) Box plots depicting the length of TNTs formed in S2013 and CAPAN-2 cells every 24 hours, up to 96 hours. The box indicates the 25th to 75th percentiles, the solid line represents the median, and the whiskers represent the minimum and maximum values. (C) High-magnification view of particularly long TNTs (TNT shown in inset exceeds 500 µm in length) connecting CAPAN-2 pancreatic carcinoma cells (magnification = 1.5 times 20x objective).
