Figure 2
From: Cysteine allows ovarian cancer cells to adapt to hypoxia and to escape from carboplatin cytotoxicity
The dose dependent and cell density dependent effects of cysteine in hypoxia adaptation in ES2 (OCCC) and OVCAR3 (OSC) cells. (A) Pearson correlation under normoxia for ES2 cell line. (B) Pearson correlation under hypoxia for ES2 cell line. (C) Pearson correlation under normoxia for OVCAR3 cell line and (D) Pearson correlation under hypoxia for OVCAR3 cell line for 16 h of assay. Treatments were normalized by the control values (normoxia without cysteine supplementation). (E) Percentage of total cell death for ES2 cells for 16 h of assay for different initial cell densities. (F) Pearson correlation for NC/N and HC/H ratios for ES2 cells. (G) Percentage of total cell death for OVCAR3 cells for 16 h of assay for different initial cell densities and (H) Pearson correlation for NC/N and HC/H ratios for OVCAR3 cells. N – Normoxia; NC – Normoxia supplemented with cysteine; H – Hypoxia; HC – Hypoxia supplemented with cysteine. 5 × 103, 2.5 × 104, 5 × 104 and 1 × 105 refers to initial cell densities expressed as cells/cm2. Results are shown as mean ± SD.
