Figure 4
From: Cysteine allows ovarian cancer cells to adapt to hypoxia and to escape from carboplatin cytotoxicity
ES2 (OCCC) adaptation to hypoxia relies on free intracellular cysteine availability. Free intracellular levels of (A) Cys– cysteine, (B) S-cysteinylated proteins – CysSSP, (C) GSH – Glutathione, (D) GluCys – Glutamylcystein, (E) CysGly – Cysteinylglycine and (F) free extracellular CysGly/free extracellular GSH ratio in ES2 (black bars) and OVCAR3 (grey bars) cells. N – Normoxia; NC – Normoxia supplemented with cysteine; H – Hypoxia; HC – Hypoxia supplemented with cysteine. Results are shown as mean ± SD. Cardinals represent statistical significance between cell lines. Asteriscs represent statistical significance among treatments within the same cell line or in comparison with the control (normoxia without cysteine supplementation). *p < 0.05, **p < 0.01, ***p < 0.001 (One-way ANOVA with post hoc Tukey tests).
