Figure 4 | Scientific Reports

Figure 4

From: Bacterial nanocellulose stimulates mesenchymal stem cell expansion and formation of stable collagen-I networks as a novel biomaterial in tissue engineering

Figure 4

Collagen-I formation by cells seeded on BNC. (a) Two-colour multiphoton live cell images with AF (grey) showing the cells and SHG (red) representing cell-derived collagen-I. MSCs were cultured in various media of which standard growth (Std) and serum-reduced media (1% and 3% FBS) with elevated ascorbic acid (aa, 4x) resulted in collagen-I fibre formation (I, blue arrows). Images below (separated AF and SHG channel) reveal the collagen fibre structure and location relative to the cells. (II) Detection of collagen formed by L929 cells grown in Std and serum-reduced media. All images are representative for five individual experiments. (b) The table presents the time-frame of collagen-I formation by MSCs with various stimulation media. SHG signals were imaged using identical laser excitation and detection conditions. Analysis was performed semi-quantitatively by defining three categories of intensity (+, ++, +++).

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