Figure 5 | Scientific Reports

Figure 5

From: Bacterial nanocellulose stimulates mesenchymal stem cell expansion and formation of stable collagen-I networks as a novel biomaterial in tissue engineering

Figure 5

Collagen-I labelling and network analysis. (a) Collagen-I networks were independently imaged by confocal fluorescence microscopy (CFM) after specific staining with CNA35-AlexaFluor546 collagen-I binding protein. Much more details of fibre network structure compared to MPM images are visible (M = 20x). (b) Comparison of collagen fibre networks at the cell-BNC interface (I) and in between cells in a multilayer (II) using a fluorescence image and the corresponding S-colour survey image (colour code expressed by colour wheel at top right). Histograms mapping the incidence of fibre orientations from −90° to +90° including values for Orientation and Coherency are shown below.

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