Figure 6

Differentiation status of MSCs. (a) MSCs were cultured on BNC or culture plate for 16 days and analysed for expression of CD31, CD34, CD45, CD73, and CD90 by flow cytometry. (I) Overlays show expression of indicated molecules (black) and corresponding isotype controls (grey, filled) at day 16 on BNC. (II) Differentiation of MSCs was analysed at day 0 (2 days after starting cell culture (white bars) and after 16 days on plastic plates (grey bars) or BNC (black bars). Graph represents mean fluorescence intensity (MFI) of surface markers relative to their isotype controls. (b) (I) AP activity (luminometry) data from MSCs seeded on BNC compared to standard cell culture plates (control) are displayed versus days after seeding. In (II), relative treatment effects, calculated based on the non-parametric model, reveal a significantly enhanced AP activity with major differences up to day 7, at which point activity already decreases for cells growing on BNC, while a similar effect takes up to day 10 for culture plate growing cells. ‘*’ Signs indicate time accumulating group differences between cells on BNC and culture plate and denote significances as follows: *p < 0.05; **p < 0.005; ***p < 0.0005.