Figure 1
From: A conditional silencing suppression system for transient expression
PLP0 putative domains and silencing suppression activity. (A) Predicted PLP0’s tertiary structure revealed two domains (blue), herein referred to as PLP0-A (N-terminal domain) and PLP0-B (C-terminal domain), separated by a linker (red). Amino acid conservation of PLP0 among PLRV isolates (bottom of panel) shows relatively low conservation of the predicted linker (red line). (B) Linker amino acid sequence (top of panel) and artificially introduced STOP (asterisk) and START (blue “M”) codons (bottom of panel) used to produce combinations of PLP0-A (PLP0 N-terminus) and PLP0-B (PLP0 C-terminus) proteins. (C) Representative N. benthamiana leaf co-infiltrated with GFP and PLP0 and each combination of PLP0-A and B (top of panel). Optical density (OD) was 0.2 per construct and analysed at 5 days post-infiltration (dpi). Fluorescence quantification of five biological replicates was performed using Fiji software and values are relative to GFP alone (bottom of panel). Pairwise comparisons (t-test; p-value < 0.05) are indicated by letters above bars; i.e., different letters indicate statistical differences in fluorescence levels.
