Figure 3
From: A conditional silencing suppression system for transient expression
Schematic of Q system components and their expression in N. benthamiana leaves. (A) Schematic representing the function of the Q system components (modified from Potter & Luo 2011). The QUAS promoter is fused to a gene of interest (GOI) and, in the presence of QF transcription factor, is active, leading to the production of a cognate protein of interest (POI). QS, however, represses QF transcriptional activity; hence, in the presence of QS, QUAS is inactive. P1 and P2 indicate promoter regions. (B) QUAS was fused to β-glucuronidase (GUS) in a binary vector constitutively expressing QF (top of panel). Agroinfiltrated N. benthamiana leaves were stained for GUS activity at the indicated bacterial optical density (OD) per construct at 6 and 10 days post-infiltration (dpi). (C) A binary vector constitutively expressing QS was used in combination with QS/QUAS/GUS vector (top of panel). Agroinfiltrated leaves were stained for GUS as indicated. Scale bars, 400 μm.
