Figure 5

Schematic of the conditional silencing suppression system, split PLP0. In the absence of the split PLP0 system (left panel), co-infiltration of two genes of interest (GOI-1 and GOI-2) results in expression of both genes at 6 days post-infiltration (dpi); however, their co-expression is only partial (i.e., a number of cells only express GOI-1 or GOI-2). Complete co-expression is obtained using the conditional silencing suppression system (right panel). Constructs expressing GOI-1 and GOI-2 are modified to also express PLP0-A2 and PLP0-B2, respectively, and are analyzed at 10 dpi, resulting in analysis of only cells that express both genes of interest.