Figure 3
Nintedanib treatment altered the gene expression of cell cycle molecules in human PCa cells after 72 h of treatment. RT2qPCR analysis using Human Cell Cycle RT2 profilerTM PCR Array (Qiagen) to assess the effect of Nintedanib on expression of genes associated with cell cycle regulation in (a) PC3, and (b) LNCaP cells. The relative quantification of gene expression between control and Nintedanib treated samples was achieved by normalization against endogenous GAPDH and β-Actin using the ΔΔCT method of quantification and the data was analyzed using the software provided by the manufacturer. The list details the up and downregulated genes in PCa cells after Nintedanib treatment.
