Figure 6

Nintedanib treatment decreased cell proliferation and microvessel density in PC3 xenograft tumors. Immunohistochemical staining of PC3 tumors for (a) Ki-67, (b) CyclinD1, (c) cleaved-caspase 3 (c-caspase 3), (d) CD31 (microvessel density), (e) Nestin, and (f) VEGF expression. Data details the comparative outcomes from both Experiment 1 and Experiment 2 study designs relative to vehicle controls. Values are expressed as % positive cells or as immunoreactivity score (arbitrary vales) as detailed in Methods section. Positively stained markers are shown by arrowhead (&#x25BA;). Representative photographs are presented at 400X; inset represents further magnification of a part of the photograph. Values are expressed as mean ± S.E.M. (n = 5) (two-tailed t-test between treated groups and its respective control). ^*p < 0.05; ^**p < 0.01; ^***p < 0.001.