Figure 1 | Scientific Reports

Figure 1

From: Expression ratio of the TGFβ-inducible gene MYO10 is prognostic for overall survival of squamous cell lung cancer patients and predicts chemotherapy response

Figure 1

TGFβ treatment triggers EMT in SK-MES1 cells. (A) TGFβ induces Smad2/3 phosphorylation in TGFβR1-dependent way. SK-MES1 cells were pretreated with TGFβR1 inhibitor SB-431542 or DMSO and then stimulated with 2 ng/ml TGFβ1. Data presented correspond to mean and SD, n is the number of independent experiments. Additional replicates are shown in Supplementary Fig. S1A. Full-length blots are shown in Supplementary Fig. S6. (B) Prolonged exposure of SK-MES1 cells to TGFβ1 induces acquisition of EMT-like morphology. Cells were either stimulated with 2 ng/ml TGFβ1 or left untreated for 3 days, fixed and stained for F-actin (white) and DNA (blue). Scale bar corresponds to 50 µm. (C) EMT marker genes are upregulated upon TGFβ1 treatment. Growth factor-depleted SK-MES1 cells were stimulated with 2 ng/ml TGFβ1 or left untreated. RNA was extracted and analyzed using qRT-PCR. mRNA expression was normalized to four housekeepers: GUSB, HPRT, GAPDH and G6PD. Each dot represents a biological replicate. A second independent experiment is shown in Supplementary Fig. S1B.

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