Figure 3 | Scientific Reports

Figure 3

From: Activation of the p53-MDM4 regulatory axis defines the anti-tumour response to PRMT5 inhibition through its role in regulating cellular splicing

Figure 3

PRMT5 inhibition causes changes in gene expression and splicing. (A) The number of significant rMATS alternative splicing events (FDR < 0.01, SD < 0.2, junction coverage > 10, −0.2 < IncLevelDifference >0.2) in lymphoma cell lines treated with 200 nM GSK3326595 for 3 days. p53 mutant cell lines are highlighted in red. (B) Significantly alternatively spliced genes were submitted for enrichment of gene sets for each cell line using MsigDB where cell cycle was one of the top gene sets for all cell lines. This cell cycle gene set compiled from all cell lines was analyzed by ReactomePA. p53 mutant cell lines are highlighted in red. (C) The number of significant gene expression changes (FDR < 0.05) in lymphoma cell lines following treatment with 200 nM GSK3326595 for 3 or 6 days. Cell lines are sorted by decreasing sensitivity based on gIC50 values in a 6-day proliferation assay. p53 mutant cell lines are highlighted in red. (D) Significantly changed genes in p53 wild-type cell lines were submitted for MsigDB enrichment analysis (Broad) and the most significantly enriched gene set for each cell line is reported.

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