Figure 5

Characterization of the QS-13 residues crucial for αvβ3 integrin interaction. SK-MEL-28 melanoma cells were pre-incubated 30 min in the presence or absence of the RGDS peptide (1 to 20 µg/mL) or anti-αvβ3 blocking antibody (20 µg/mL) and adhesion was assessed. *p < 0.05, ***p < 0.01, ***p < 0.001 (a). SK-MEL-28 melanoma cells were incubated with different substituted peptides (b) tagged with biotin and with AF-488 streptavidin complex and analyzed by flow cytometry (c) and results are reported on a histogram (d).