Figure 1

Visualization of SFTA3. (A) Specific detection of SFTA3 mRNA in the following samples: lacrimal gland, eyelid, conjunctiva, cornea, human corneal epithel cells (HCE), human conjunctival epithel cells (HCjE). Samples without cDNA were used as negative controls, whereas samples containing lung cDNA were used as positive controls. (B) Western blot analysis with a polyclonal anti-rabbit SFTA3 antibody incubated on protein extracts derived from tear film [1], tear film [2], HCE [3], HCjE [4] and HMGEC [5] (C) Immunohistochemical detection (red staining, black arrows) of SFTA3 in tissues of the ocular surface, lacrimal glands and eyelids. [a] Eyelid: detection in the basal epithelium of the eyelid. [b] Conjunctiva: multilayered epithelium of the conjunctiva shows weak reactivity. [c,d] Meibomian glands and Glands of Moll: SFTA3 detected within the cytoplasm of acinar cells and cells of the excretory duct system. [e,f] Lacrimal gland: detected in secretory ducts. [g] Cornea: protein detected in the basal epithelium. (s = stroma, e = epithelium, b = basal) [h] Cornea endothelium: positive staining in the endothelial cells. (D) HCE and HCjE cell lines: detection of SFTA3 within the plasma membrane (SFTA3: green; DAPI (4′,6-diamidino-2-phenylindole): blue).