Figure 5 | Scientific Reports

Figure 5

From: SFTA3 – a novel surfactant protein of the ocular surface and its role in corneal wound healing and tear film surface tension

Figure 5

Influence of cytokines on the expression of SFTA3 in the cell lines HCE and HCjE. Stimulation of HCE cells (A,B) for 6; 12; 24 and 48 hours with 10 ng/ml of recombinant IL-1β or TNFα, respectively. Statistical significance of the decrease in SFTA3 mRNA expression after 6 and 12 hours for IL-1β (n = 3; *P ≤ 0.05, **P ≤ 0.005) and after 24 hours for TNFα (n = 3; *P ≤ 0.05). (A) Relative quantification of SFTA3 mRNA by Real Time PCR. (B) Relative quantification of SFTA3 protein by ELISA showed no statistically significant differences. Stimulation of HCjE cells (C,D) for 6; 12; 24 and 48 hours with 10 ng/ml of recombinant IL-1β or TNFα, respectively. Statistically significant decrease in SFTA3 mRNA expression after 24 and 48 hours for IL-1β and TNFα (n = 3; *P ≤ 0.05). (C) Relative quantification of SFTA3 mRNA by Real Time PCR. (D) Relative quantification of SFTA3 protein by ELISA. Statistically significant reduction of SFTA3 protein after 48 hours for TNFα (n = 3; *P ≤ 0.05).

Back to article page