Figure 2
From: HMGB2 is a novel adipogenic factor that regulates ectopic fat infiltration in skeletal muscles
Hmgb2 deficiency reduced MSC adipogenic differentiation. (a) Quantitative PCR was performed to assess the Hmgb2, Pparg and Cebpa levels in WT and Hmgb2−/− MSCs during adipogenesis. (b) The expression levels in the cells at each time point in three independent experiments were determined by Western blotting of WT and Hmgb2−/− MSCs during adipogenesis. The exposure time for HMGB2, PPARG and CEBPA was 120 sec, and the time for β-actin was 2 sec. HMGB2/β-actin and PPARG/CEBPA were detected in the different part of the each gel, respectively. Representative data from three separate experiments are shown. (c) Quantitative PCR in MSCs transfected with Hmgb2 siRNA. (d) Oil-red O staining of WT MSCs, MSCs transfected with Hmgb2 siRNA, and Hmgb2−/− MSCs 7 days after adipogenic induction (original magnification, x400, 0.237 mm2/view). (e) Percentage of cells positive for lipid droplets among the WT and Hmgb2−/− cells on day 7. n = 3 for each time point. *p < 0.001.
