Figure 3
From: HMGB2 is a novel adipogenic factor that regulates ectopic fat infiltration in skeletal muscles
HMGB2 was co-expressed with PDGFRA in undifferentiated MSCs, and Hmgb2 deficiency was associated with PDGFRA reduction. (a) Double immunofluorescence representing HMGB2 and PDGFRA expression in MSCs 1 day after adipogenic induction (original magnification, x630). (b) Flow cytometry analysis for the expressions of PDGFRA and Sca-1 during adipogenesis of MSCs. Representative data from three different experiments are shown. (c) The percentages of PDGFRA+, Sca-1+, and PDGFRA+/Sca-1+ cells in WT (black lines) and Hmgb2−/− MSCs (red lines) are plotted. n = 3 for each time point.
