Figure 6 | Scientific Reports

Figure 6

From: Mechanistic insights into the augmented effect of bone marrow mesenchymal stem cells and thiazolidinediones in streptozotocin-nicotinamide induced diabetic rats

Figure 6

(A) Representative H&E-stained cardiac sections of the different groups (400x). Myocardium of rats of the control groups showing normal branching of myocardial fibers with acidophilic cytoplasm and centrally located, oval vesicular nuclei. Myocardium of diabetic rats showing inflammation (i), eosinophilic staining (e) and degeneration (d) in myocardial structure. Myocardium of rats of diabetic rats-treated with MSCs and/or PIO group showing minimal inflammation, eosinophilic staining and degenerations. (B) Representative IHC images showing expression of caspase 3 in the myocardium (400x). Intensity of dark brown staining indicates sites where caspase-3 level is expressed and from which the caspase 3-positive area were quantitatively analyzed. (C) The cardiac protein expression of Bcl-2, Bax and cleaved caspase 3 were examined by Western blotting assay. (D) RT-PCR analysis of the expression of Na-K-ATPase at mRNA level in each group. Data are presented as Mean ± s.e.m. *P < 0.05 vs. control group; a P < 0.05 vs. diabetic group; b P < 0.05 vs. Diabetic + MSCs group.

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