Table 2 Gene-Specific Primers Used for RT-PCR.
Gene symbol | Gene ID | Primer sequence 5–3 |
|---|---|---|
PGC-1α | NM_008904 | F 5′-AAACTTGCTAGCGGTCCTCA-3′ R 5′-TGGCTGGTGCCAGTAAGAG-3′ |
CPT1a | NM_013495 | F 5′-TGGTCAACAGCAACTACTACGC-3′ R 5′-GAAGACGAATGGGTTTGAGTTC-3′ |
PPARα | NM_011144 | F 5′-CAACGGCGTCGAAGACAAA-3′ R 5′-TGACGGTCT CCACGGACAT-3′ |
GLP-1R | NM_021332.2 | F 5′-ACTTTCTTTCTCCGCCTTGGT-3′ R 5′-CCTGGTGCAGTGCAAGTGTCT-3′ |
SREBP-1c | NM_009204.2 | F 5′-CTGGCACTAAGTGCCCTCAAC-3′ R 5′-GCCACATAGATCTCTGCCAGTGT-3′ |
GLUT4 | NM_009204.2 | F 5′-TCGTCATTGGCATTCTGGTTG-3′ R 5′-AGCTCGTTCTACTAAGAGCAC-3′ |
IRS-1 | NM_010570 | F 5′-GCCAATCTTCATCCAGTTGC-3′ R 5′-CATCGTGAAGAAGGCATAGG-3′ |
IRS-2 | NM_001081212 | F 5′-CATCGACTTCCTGTCCCATCA-3′ R 5′-CCCATCCTCAAGGTCAAAGG-3′ |
Na+-K+-ATPase | NM_001160234.1 | F 5′-CCTGGGAGGCTTCTTCACTT -3′ R 5′-CTGCTCGTAGGTCCACTGCT-3′ |
Collagen I | NM_007742 | F 5′-CAACCTGGACGCCATCAAG -3′ R 5′-CAGACGGCTGAGTAGGGAACA-3′ |
Collagen III | NM_009930 | F 5′-TTGATGTGCAGCTGGCATTC-3′ R 5′-GCCACTGGCCTGATCCATAT-3′ |
TGFβ1 | NM_011577 | F 5′-ATGCTAAAGAGGTCACCC-3′ R 5′-CAAAAGACAGCCACTCAG-3′ |
βActin | NM_031144.3 | F 5′-TATCCTGGCCTCACTGTCCA-3 R 5′-AACGCAGCTCAGTAACAGTC-3 |
