Figure 2 | Scientific Reports

Figure 2

From: Spontaneous reactivation of latent HIV-1 promoters is linked to the cell cycle as revealed by a genetic-insulators-containing dual-fluorescence HIV-1-based vector

Figure 2

Longitudinal fluorescence profiles of transduced SUP-T1 cell populations. (a) SUP-T1 cells were transduced with LTatC[M] (squares) or a vector variant with no genetic insulators in the mCherry cassette (LTatCM) (triangles). Double positive (DP), single mCherry positive (M+), and double negative (DN) cell populations were sorted 10 days post transduction and their fluorescence profiles were measured with flow cytometry for 191 days post transduction. Shaded symbols depict percentages of cells with the fluorescence initially sorted for whereas open symbols depict percentages of cells that became DN and M+. Each datapoint of the DP data set represents the mean of duplicate cultures (n = 2) and error bars depict standard error means. Some error bars are within datapoints. (b) Longitudinal fluorescence profiles of sorted SUP-T1 cell populations up to 394 days post transduction in a second transduction experiment.

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