Figure 1

UCM-MSCs have a higher proliferation rate than UCB-MSCs. (A) Morphological aspect of UCM-MSCs and UCB-MSCs during monolayer cell culture at P3 observed by phase-contrast microscopy (magnification × 10). (B) Samples characteristics. This table recapitulates the number of independent cryopreserved UCM- and UCB-MSCs vials received for analysis, and the number of samples evaluated for each passage. (C) Proliferation characteristics of UCM- and UCB-MSCs upon successive passages. This graph represents the doubling time (h) of UCB-MSCs and UCM-MSCs cultured up to P13. Samples with doubling time above 400 h were systematically excluded from the representation, these samples being considered as non-proliferating. A statistical analysis was realized to compare the proliferation potential of UCM- and UCB-MSCs using multiple comparison two-way ANOVA test (**p < 0.01) when the number of samples was appropriated (from P2 to P7). (D) Flow cytometry graph analysis of representative samples of UCM-MSCs and UCBM-MSCs. Recapitulative table of mean values and standard deviation is presented for CD-45, MHC-1, MHC-2, CD-90, CD-29 and CD-44. (E) UCB-MSCs and UCM-MSCs multilineage évaluation. UCM-MSCs and UCB-MSCs were induced in adipogenic, osteogenic and chondrogenic lineage by using specific induction media. ECM mineralization was demonstrated by alizarin red S staining. Lipid droplets in the cytoplasm are highlighted by oil red O staining and sulfated matrix observed during chondrogenesis was evidenced by alcian blue staining. A representative example from seven samples for UCM-MSCs and five samples for UCB-MSCs are shown (magnification: ×10 and ×20).