Figure 2 | Scientific Reports

Figure 2

From: Differences in the intrinsic chondrogenic potential of equine umbilical cord matrix and cord blood mesenchymal stromal/stem cells for cartilage regeneration

Figure 2

Strong differences between UCM-MSCs and UCB-MSCs in gene expression during chondrogenesis. Chondrogenesis was induced in UCM-MSCs and UCB-MSCs by culturing the cells in 3D scaffolds under BMP-2 and TGF-ß1 treatment. The time-course monitoring of chondrogenic (Col2a1, Acan, Sox9), hypertrophic (Mmp13, Col10a1) (upper panel) and osteogenic markers (Col1a1, Alpl, Htra1, Spp1, Runx2 and Bglap) (lower panel) were assessed at 7, 14, 21 and 28 days post-treatment. At each time point, the mRNA levels of each transcript were normalized to the mRNA levels of the housekeeping gene. Relative expression of each gene was compared to the expression of the control (D0) set at 1. Small dots represent the distribution of the values measured for the 5 independent samples at each time. The images below the graphs represent the macroscopical appearance of the neo-tissue substitute following 28 days under the chondrogenic regimen (BT treatment).

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