Figure 4 | Scientific Reports

Figure 4

From: Differences in the intrinsic chondrogenic potential of equine umbilical cord matrix and cord blood mesenchymal stromal/stem cells for cartilage regeneration

Figure 4

Protein analysis of UCM-MSCs and UCB-MSCs after chondrogenic differentiation. UCM-MSCs and UCB-MSCs were cultured in type I/III collagen scaffolds in normoxia during 7, 14, 21 and 28 days in ICM supplemented with BMP-2 (50 ng/ml) and TGF-ß1 (10 ng/ml). Protein extracts were analyzed in western-blots for type II collagen, type I collagen and Htra1. Representative blots of UCM-MSCs (n = 5) (A) and UCB-MSCs (n = 3) (B and C) are shown. (C) 2.5 µg of protein extracts were used for the western-blot of UCB-MSCs samples in order to enhance signal accuracy. The blots presented come from 2 independent experiments and the figure has been composed from 3 western-blots, each coming from one gel. Different levels of type II and type I collagen maturation forms are indicated such as type II procollagen (pro), without C- or N- terminal propeptides (Pc/Pn) and the mature doubly cleaved form (mat).

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