Figure 6

Comparison of the osteo-chondrogenic markers expression between UCM-MSCs and UCB-MSCs in longitudinal study. UCM-MSCs and UCB-MSCs were cultured in monolayer to sub-confluence. Steady-state levels of mRNA expression in UCB-MSCs and UCM-MSCs were compared by RTqPCR. All results were normalized versus undifferentiated sample of one UCM-MSCs sample, and presented as the relative expression of each gene. Box-plots represent five independent experiments and statistically significant differences were determined using the Mann Whitney test by comparison of UCM-MSCs versus UCB-MSCs *: *p < 0.05, **p < 0.01. (B) Four samples of monolayer cultured MSCs for each MSCs sources (numbers 1 to 4) were compared at protein level by western-blot analysis of types I and II collagens, osteocacin, Sox-9 and Runx-2. The images presented correspond to a western-blot including 2 gels.