Figure 2
(a) Mutability landscape of ADS for catalytic activity using bioluminescent assay. (b) Mutability landscape of ADS for catalytic activity using GC-MS assay. For the mutability landscapes, the vertical axis portrays the 20 possible amino acid residues. The wild-type amino acid residue at each position is indicated by bold squares, white squares represent variants that are not present in the library and grey squares represent variants that are not expressed. The color represents the catalytic rate of reaction (V) where blue range squares indicate decrease in catalytic activity while red range squares indicate increase in catalytic activity compared to the wild type. The illustrated data are an average of two separate experiments (n = 2). (c) Multiple sequence alignment of the mutability landscape of ADS with ten sesquiterpene synthases and three monoterpene synthases. Residues that are identical, strongly similar, weakly similar and non-matching compared to ADS are colored dark blue, blue, cyan and white, respectively.
