Table 1 Steady state kinetic parameters of wild type ADS and selected variants with FPP as the substrate.

From: Catalysis of amorpha-4,11-diene synthase unraveled and improved by mutability landscape guided engineering

Enzyme

kcat (s−1)

Km (μM)

kcat/Km (s−1.M−1)

ADSWT

0.20 ± 0.007

5.47 ± 0.558

3.6 × 104

ADSH448A

0.68 ± 0.03

5.04 ± 0.753

13.5 × 104

ADST399S

0.39 ± 0.06

5.89 ± 1.278

6.6 × 104

ADST399S/H448A

1.00 ± 0.14

8.50 ± 1.605

11.8 × 104

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