Figure 1
(A) Double fluorescence switch strategy for visualizing fusion. Preliminary tests of the fluorescence labelling method showed it to be a viable effective research tool. (A,F) A schematic presentation of the experiments. (B–E) Raw264.7 cells were treated with RANKL to produce fusion. (G–J) BMMs from Ctsk-Cre and RosamTmG were 1:1 co-cultured and induced with an osteoclastogenic medium. Images from fluorescence microscope via this fluorescence switch strategy clearly and concisely presented fused GFP+ cells (G,I). In contrast to this strategy, the images produced by the traditional bright field method lose much information and make it difficult to specifically observe fused cells (H&J). All conclusions presented here reliably represent the results of experiments carried out at least three times. Scale bar: 50 um.
