Figure 2
UHI microscopy of a live neutrophil. (a) Optical density (OD) map of an unstained, isolated human neutrophil at 260 nm wavelength with inset depicting a bright-field microscopy image of the same neutrophil after Giemsa staining. (b) Nucleic acid mass map of a neutrophil with an average mass of 23.81 fg within the cell. (c) Protein mass map of a neutrophil with an average mass of 273.65 fg within the cell. (d) OPL map of a neutrophil at 260 nm. (e) OPL spectra averaged over cell nucleus, nucleus periphery, and cytoplasm. (f) Optical density spectra extracted from cell nucleus, nucleus periphery, and cytoplasm. (g) Phasor space representation of the attenuation spectra obtained from the imaged neutrophil. Dotted black lines outline the unit circle and universal semicircles. (h) Molecular image obtained by color coding the phasor space clusters based on the color bar shown in inset of (g).
