Figure 4

Levels of FcNAC1 transcripts in different F. chiloensis tissues. Quantification of transcripts was performed by qRT-PCR employing specific primers: (A) during development and ripening of Fragaria chiloensis fruit (C1, C2, C3 and C4); (B) different plant tissues (leaves, flowers, roots, stem and runners). RNA extractions from the samples were performed using the CTAB method. FcGAPDH was used as internal calibrator gene. Different letters indicate statistical difference between fruit stages (A) or plant tissues (B). Significant differences were determined at p ≤ 0.05 (LSD Fisher test). In order to facilitate the comparison of transcripts level, C1 fruit stage in A, and leaf in B were normalized to 1. All pictures were taken by one of the authors.