Figure 5
From: Characterization of a ripening-related transcription factor FcNAC1 from Fragaria chiloensis fruit
Effect of hormones on the transcription of FcNAC1. (A) Expression changes of FcNAC1 in response to ABA and auxins. F. chiloensis fruit at the C2 stage was subjected to hormonal treatments (1 mM ABA and 1 mM ANA) or immersed in buffer (control). Samples were collected just after treatment (10 min), and after 1, 2 and 12 h, and stored at −80 for subsequent RNA extractions by the CTAB method. Expression analyses were performed through qRT-PCR. For the same treatment, different letters indicate significant differences during the observation period. Significant differences were determined at p ≤ 0.05 (LSD Fisher test). (B) Analysis of FcNAC1 promoter. By means of the genome Walker technique, a sequence of 1488 bp corresponding to the promoter sequence of FcNAC1 was obtained and in silico analyzed with PlantCARE database (http://bioinformatics.psb.ugent.be/webtools/plantcare/html/). Different putative cis-elements related to several hormonal responses were identified along the sequence.
